Demonstration of basement membrane in renal biopsies by silver-methenamine on thin epoxyresin sections.

In the recent paper on an improved technique for renal biopsies, Meadows and Schoemaker (1970) describe the use of a special wax to give fine sections at 1-2,.u. They illustrate their excellent results with Jones's silver technique. In this laboratory, however, difficulty was experienced with their use of ammoniacal ethanol and as we, in agreement with Eastham and Essex (1969), believe that the thinner sections possible with polymer-embedding are of greater diagnostic value, the present note describes the successful application of a methenamine-silver method on sections from Araldite.